Core Facilities: Microscopy & Digital Imaging

Eric Pearlman, Ph.D., Module Director Departments of Ophthalmology and The Center for Global Health and Diseases phone #368-1856 eric.pearlman@case.edu Scott Howell, Ph.D., Module Manager Department of Ophthalmology phone #368-2300 scott.howell@case.edu

Located in The VSRC Consolidated Laboratory Space In the Institute of Pathology
The imaging module has been divided into two major areas to better meet the needs of researchers. The two areas are Digital Imaging and Microscopy Based Digital Imaging. This division of this module allows the personnel involved to utilize their areas of expertise to the fullest extent. Researchers and their laboratory personnel can schedule training sessions or receive direct assistance from the module manager.

The module manager maintains protocols to aid in training and use of all module equipment. The module manager works with the other modules to meet the imaging needs of investigators using those facilities.

Digital Imaging Module
The purpose of this division is to provide advice, guidance and training in the management of digital images, as well as to provide highly specialized ophthalmic photography services. This division assists Core Grant investigators in producing scientific posters and publication quality graphics.

Microscopy Based Imaging Module
This division of the module was created to meet the ever increasing demand of microscopy based services by Core Grant investigators. The VSRC has invested heavily in this division of the module with an emphasis on being able to provide Core Grant investigators access to experienced personnel and state of the art equipment. One strength of the core is to provide image analysis to save time and money for investigators with highly reproducible results. Additionally, Metamorph image analysis software is being used in the module to not only save time and resources but also to generate reproducible results. Please refer to the Microscope Policy to best utilize this module.

Equipment & Services Available

Digital Imaging Module:
* -Digital Graphics Production
* -Animal Slit Lamp Photography
* -Poster and Publication Production
* -Digital Photography Equipment Usage
* -High Resolution Flatbed/Transparency Scanning
* -Imaging Computer Workstation Usage

Microscopy Based Imaging Module:
Please refer to the Microscope Policy to best utilize this module.
* -Image Analysis using Metamorph Imaging Software. VSRC has three copies of software for VSRC member usage. Module Manager can assist in developing time saving macros to quantify fluorescence, measure areas and lengths, count cells, etc.
* -Training on Microscopes and Imaging Software.
* -Imaging Based Experimental Design
* -Olympus BX-60 Upright Microscope with DIC and Fluorescence attached to a BW/Color SPOT RT Slider Camera (Objectives 2x, 4x, 10x, 20x, 40x and 100x oil)
* -Leica DMI 6000 B Automated Inverted Microscope with Phase Contrast, Fluorescence, Motorized Stage and Z Focus. Microscope is attached to a BW/Color Retiga EXi camera and the entire system is controlled by Metamorph Software. (Objectives 10x, 20x, 40x and 63x High NA oil) detailed description here
* -Laser Capture Microdissection (LCM) in cooperation with the Case Comprehensive Cancer Center. Imaging Module manager is chief trainer on the Arcturus Veritas LCM and VSRC members are afforded discounted usage rates.
* -FRET ability to do CFP/YFP FRET
* -heated stage for short timelapse studies in HEPES no CO2

Online Sign Up for Equipment
Microscope Policy
Sign up for equipment will make use of Yahoo Calendar
To schedule time on the Leica DMI 6000 B Inverted Scope Click HERE http://calendar.yahoo.com/
User name is invertedscope
Password is microscope
To schedule time on the Olympus BX-60 Upright Scope Click HERE http://calendar.yahoo.com/
User name is slidemicroscope
Password is slidesonly