Eric Pearlman, Ph.D., Module Director
Departments of Ophthalmology and
The Center for Global Health and Diseases
phone #368-1856
Beth Ann Benetz, M.A., CRA, Module Co-Director
Department of Ophthalmology
phone #844-3615
Scott Howell, Ph.D., Module Manager
Department of Ophthalmology
phone #368-2300
Located in The VSRC Consolidated Laboratory Space In the Institute of
Pathology
The imaging module has been divided into two major areas to better
meet the needs of researchers. The two areas are Digital Imaging and
Microscopy Based Digital Imaging. This division of this module allows
the personnel involved to utilize their areas of expertise to the fullest
extent. Researchers and their laboratory personnel can schedule training
sessions or receive direct assistance from the module manager.
The module manager maintains protocols to aid in training and
use of all module equipment. The module manager works with the
other modules to meet the imaging needs of investigators using
those facilities.
Digital Imaging Module
The purpose of this division is to provide advice, guidance and
training in the management of digital images, as
well as to provide highly specialized ophthalmic photography services.
This division assists Core Grant investigators in producing scientific
posters and publication quality graphics. Additionally a Certified
Retinal Angiographer is available to provide imaging of the anterior
and posterior segments of both large and small animals. This photographer
also provides general photography services to Core
Grant investigators.
This division of the module was created to meet the ever increasing
demand of microscopy based services by Core Grant
investigators. The VSRC has invested heavily in this division
of the module with an emphasis on being able to provide Core Grant
investigators access to experienced personnel and state of the
art equipment to meet not only live cell imaging needs but also
basic microscopy needs as well. Additionally, Metamorph image
analysis software is being used in the module to not only save
time and resources but also to generate reproducible results. Please refer to the Microscope Policy to best utilize this module.
Equipment & Services Available
Digital Imaging Module :
- -Digital Graphics Production
- -Animal Fundus Photography and Angiography
- -Animal Slit Lamp Photography
- -Poster and Publication Production
- -Fuji Pictography Printing
- -Digital Photography Equipment Usage
- -High Resolution Flatbed/Transparency Scanning
- -Imaging Computer Workstation Usage
Microscopy Based Imaging Module:
Please refer to the Microscope Policy to best utilize this module.
- -Image Analysis using Metamorph Imaging Software.
VSRC has three copies of software for VSRC member
usage. Module Manager can assist in developing
time saving macros to quantify fluorescence,
measure areas and lengths, count cells, etc.
- -Training on Microscopes and Imaging Software.
- -Imaging Based Experimental Design
- -Olympus BX-60 Upright Microscope with DIC
and Fluorescence attached to a BW/Color SPOT
RT Slider Camera (Objectives 2x, 4x, 10x, 20x,
40x and 100x oil)
- -Leica DMI 6000 B Automated Inverted Microscope
with Phase Contrast, Fluorescence, Motorized
Stage and Z Focus. Microscope is attached to
a BW/Color Retiga EXi camera and the entire system
is controlled by Metamorph Software. (Objectives
10x, 20x, 40x and 63x High NA oil) detailed
description here
- -Laser Capture
Microdissection (LCM) in cooperation with the
Case Comprehensive Cancer Center. Imaging Module
manager is chief trainer on the Arcturus Veritas
LCM and VSRC members are afforded discounted
usage rates.
Online Sign Up for Equipment
Microscope Policy
Sign up for equipment will make use of Yahoo Calendar
To schedule
time on the Leica DMI 6000 B Inverted Scope Click
HERE http://calendar.yahoo.com/
User name is invertedscope
Password is microscope
To schedule time on the Olympus BX-60 Upright Scope Click HERE
http://calendar.yahoo.com/
User name is slidemicroscope
Password is slidesonly |
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