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Director of the Center for RNA Molecular Biology |
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Education: Ph.D. Molecular Biology, SUNY Albany, 1978 |
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Secondary Appointment in: Department of Biochemistry, Department of Medicine |
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Editor of the RNA Journal |
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Email: twn Office Location: Wood Blgd. W127 Office Phone: 216-368-1606 Office FAX: 216-368-2010 Laboratory Location: W123, RT100 K, L Laboratory Phone: 216-368-8531 |
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Our lab is currently studying several aspects of post-transcriptional gene regulation: 1. Identification and mechanistic characterization of splicing silencers. Splicing silencers are RNA elements that downregulate the use of splice sites.
2. Mechanism of trans-splicing in a parasitic nematode. We have developed cell free systems to analyze this unusual splicing reaction that joins two independently transcribed RNAs.
3. Mechanism whereby miRNAs regulate translation. miRNAs are small RNAs that bind to the 3' UTRs of target mRNAs and downregulate their translation.
4. Mechanism by which the exon junction complex enhances translation. The EJC is deposited on mRNAs as a consequence of splicing and upregulates translation of spliced mRNAs. |
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Pubmed Link to Publications Google Scholar Link to Publications
Select Publications Nilsen, T.W. and Graveley, B.R. (2009) Expansion of the eukaryote proteome by alternative splicing (Nature Insight) Nature, In Press. Yu, Y., Maroney, P.A., Denker, J.A., Zhang, X.H-F, Dybkov, O., Lührmann, R. Jankowsky, E., Chasin, L.A. and Nilsen, T.W. (2008) Dynamic Regulation of Alternative Splicing by Silencers that Modulate 5´ Splice Site Competition. Cell, 135:1224-1236. Nilsen, T.W. (2007) RNA 1997–2007: A Remarkable Decade of Discovery. Molecular Cell 28: 715-720 Maroney, P.A., Yu, Y. Fisher, J. and Nilsen, T.W. (2006) Evidence that microRNAs are associated with translating messenger RNAs in human cells. Nature Structural & Molecular Biology, 13:1102-1107. Fairman, M.E., Maroney, P.A., Wang, W., Bowers, H.A., Gollnick P., Nilsen, T.W. & Jankowsky, E. (2004) Protein displacement by DExH/D “RNA helicases” without duplex unwinding. Science 340:730-734 |
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