Lan Zhou, M.D., Ph.D.

Assistant Professor

Mailing Address:
2103 Cornell Rd.
WRB 6527
Cleveland, OH 44106-7288

phone: (216) 368-1671
fax: (216) 368-0494
email: Lan.Zhou@Case.edu

Biography
Dr. Lan Zhou earned her M.D. from Shanghai Medical University in 1991. She then spent two years at the Graduate School of Shanghai Medical University, Department of Biochemistry. The next six years were spent at State University of New York at Buffalo, NY, where she received her Ph.D. in Molecular and Cell Biology. She then worked as a Postdoctoral Fellow with Dr. John Lowe in the Howard Hughes Medical Institute, University of Michigan. She started her Clinical Pathology Residency training in 2001 followed by Transfusion Medicine Fellowship training at University of Michigan Hospitals. In 2005, Dr. Zhou came to the Case School of Medicine where she has an Assistant Professorship in the Department of Pathology. Dr. Zhou also has clinical services in Transfusion Medicine and is the Director of the Molecular Diagnostic Laboratory at University Hospitals Case Medical Center (UHCMC). Dr. Zhou is board certified in American Board of Clinial Pathology/Transfusion Medicine and American Board of Molecular Genetic Pathology.

Research
The research in my laboratory is devoted to understanding how fucosylation affects Notch-dependent regulation of hematopoietic stem cell proliferation, early myeloid lineage specification and committed neutrophil proliferation and differentiation. Active projects include: 1) characterization of the banormal hematopoietic phenotypes, particularly myeloproliferative phenotypes in mice deficient in selectin ligand activity as a result of lacking alpha (1,3)-fucosyltransferase, in mice deficient in FX protein, an enzyme essential for GDP- fucose synthesis, and in mice deficient in Notch receptor O- fucose. 2) determination of the role of Notch in hematopoietic stem cell proliferation and self-renewal, and myeloid versus lymphoid lineage specification. 3) characterization of Notch in myeloid development and its regulation by O-fucosylation. 4) characterization of bone marrow specific niche for hematopoietic stem cells. 5) studying the role of Notch and its interaction with other signaling pathways in pancreatic cancer initiation and progression. Our clinical related research focuses on the application of using real-time PCR in prenatal RhD genotyping from fetal DNA present in the plasma from D- negative pregnant women, and application of molecular diagnostic assays in infectious diseases and solid tumors and hematologic neoplasms.

Publications
Zhou, L., Yang, X.-P., Chen, H.-L. (1996) Blocking effect of retinoid acid on n-nitrosodiethylamin induced rat liver carcinoma. Chinese Biochemical Journal. 12(1): 104-108.

Zhou, L., Zhang, Z. (1997) Isolation and purification of gap junction proteins from various tissues. In Zhang, J. (Ed), Methods in Advanced Pharmacology. Beijing Medical University and Peking Union Medical University. Beijing, P.R.China.

Foote, C. R., Zhou, L., Zhu, X., Nicholson, B. J. (1998) The pattern of disulfide linkages in the extracellular loop regions of connexin 32 suggests a model for the docking interface of gap junctions. Journal of Cell Biology. 140 (5): 1187-1197.

Nicholson, B.J., Zhou, L., Cao, F., Zhu, H., Chen, Y. (1998) Diverse molecular mechanisms of gap junction channel gating. Gap Junctions. 3-7. Werner, R. (Ed) IOS press.

Zhou, L., Kasperak, E.M., Nicholson, B.J. (1999) Dissection of the molecular basis of pp60v-src induced gating of connexin 43 gap junctions. Journal Cell Biology. 144 (5): 1033-1045.

Skerrett, I.M., Merritt, M., Zhou, L., Zhu, H., Cao, F., Smith, J.F., Nicholson, B.J. (2001) Applying the Xenopus oocyte expression system to the analysis of gap junction proteins. Methods Mol Bio. 154:225-249.

Smith, P. L., Myers, J. T., Rogers, C. E., Zhou, L., Petryniak, B., Becker, D. J., Homeister, J. W., Lowe, B. L. (2002) Conditional control of selectin ligand expression and global fucosylation events in mice with a targeted mutation at the FX locus. Jourmal of Cell Biology. 158(4):801-805.

Hoffmann, S, Zhou, L, Gu, Y, Davenport, R, and Cooling, L. Delayed platelet engraftment in group O patients after autologous progenitor cell transplantation. Transfusion. 2005 Jun; 45(6):885-95.

Zhou, L, Giacherio, D, Cooling, L, and Davenport, RD. Use of B-natriuretic peptide as a diagnostic marker in the differential diagnosis of transfusion-associated circulatory overload. Transfusion. 2005 Jul;45(7):1056-63.

Zhou, L. and Schmaier, AH. Platelet aggregation testing in platelet-rich plasma: description of procedures with the aim to develop standards in the field. Am J Clin Pathol. 2005 Feb;123(2):172-83.

Zhou, L, Thorson, JA, Nugent, C, Davenport, RD, Butch, SH, Judd, WJ. Noninvasive prenatal RHD genotyping by polymerase chain reaction using plasma from D-negative pregnant women. Am J Obstet Gynecol. 2005; 193: 1966-71.

Zhou, L, Wei, LL, Yan, Q, Petryniak, B, Man, Y, Su, C, Shim, J, Chervin, S, Lowe, JB. Notch-dependent control of myelopoiesis is regulated by fucosylation. Blood. 2008 Jul 15;112(2):308-19.

Yan, Q,, Yao,D., Wei, L., Zhang, L., Xin, W.,Shin, J. Man, Y., Gerson, S., Lowe, J., and Zhou, L. O-fucosylation Modulates Notch-Controlled Lineage Commitment from Mouse Bone Marrow Hematopoietic Stem Cells and Embryonic Stem Cells. 2009 (in revision).

Yan, Q., Yao, D., Li, L.W., Zhang, L., Xin, W., Petryniak, B, and Zhou, L. (2009) Pofut1 regulates Notch-dependent control of myelopoiesis and lymphopoiesis (in preparation).