Molecular Biology Core

About
The Molecular Biology and Genotyping (MBG) Module facilitates the use of fundamental techniques in molecular biology for the many vision investigators who desire to add these tools to their research capabilities, as well as for investigators who already use some of these techniques. The MBG Module is responsible for providing skilled technical expertise, equipment and techniques for PCR analysis/genotyping, quantification and imaging of gels, real time PCR, cloning and mutagenesis, clone analysis, RNA purification and syntheses, and detailed sequence analysis. The module also provides training and assistance for the Li-Cor Odyssey Fc Infrared Imaging System for protein Westerns.

The module is currently supporting 13 investigators while genotyping approximately 100 strains of mice. Since 2012 the module has processed approximately 1,100 samples per month.


How to Utilize
1) Please call John Denker at 368-4990 to discuss if you are a first time user


2) Fill out Genotyping Request Form and email it to john.denker@case.edu or bring in with samples


Pricing Information
Genotyping: $2.50/PCR reaction
qPCR: $20.00/run
Other procedures: $15/hour plus reagents at cost

Akiko Maeda, Ph.D., Module Director
Department of Ophthalmology and Visual Sciences
2085 Adelbert Road
Institute of Pathology, Room 115
Phone: (216) 368-0670
akiko.maeda@case.edu

John Denker, Module Manager
Department of Ophthalmology and Visual Sciences
2085 Adelbert Road
Institute of Pathology Building, Room 110
Phone: (216) 368-4990


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