Janos
Kerner, PhD
Associate
Professor
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office:
SOM W165
lab:
SOM W161
phone: (216) 368-5358
fax: (216) 368-6644
email: janos.kerner@case.edu
mailing address:
Department of Nutrition
Case Western Reserve University
School of Medicine • WG 48
10900 Euclid Avenue
Cleveland OH 44106-4954
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A little
background
Janos Kerner
graduated from Humboldt University in Berlin (Germany) where he
received his M.S. in biology/plant biochemistry. He accepted a teaching/research
position in the Department of Biochemistry at Medical University
of Pecs (Hungary) where he received his Ph.D. in biochemistry. He
held a faculty position at Medical University at Pecs until 1994.
During his faculty appointment in the Department of Biochemistry
at the Medical University of Pecs, he worked for several years as
a visiting faculty in the Department of Biochemistry at Michigan
State University. He joined the faculty in the Department of Nutrition
at CWRU in 1995.
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RESEARCH
INTERESTS
My research
interest has been the elucidation of the functions of carnitine acyltransferases
and acylcarnitines in intermediary metabolism and the investigation
of the regulation of mitochondrial malonyl-CoA sensitive carnitine
palmitoyltransferase (CPT-I).
During my past research I was interested in method and assay developments for
identification and quantitation of individual acylcarnitines. Since then
I have shown that carnitine is involved in specific detoxification reactions,
and in modulating the acyl-CoA/CoASH ratio in cellular compartments. My current
research is in the area of mitochondrial fatty acid oxidation with special emphasis
on the malonyl-CoA sensitive carnitine palmitoyltransferase. CPT-I exists in
two isoforms, the liver and muscle specific isoform, and catalyzes the rate controlling
step of mitochondrial fatty acid oxidation.
Consequently, its regulation (allostericaly by malonyl-CoA and covalently
by phosphorylation) is central to several pathological conditions. Our aim
is to elucidate how the two isoforms are regulated in vivo under physiological
and pathological conditions using a multifaceted approach which involves enzymological
and immunological methods as well as proteomics.
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PUBLICATIONS
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Chandler
MP, Kerner
J, Huang H, Vazquez E, Reszko A, Martini WZ, Hoppel
CL, Imai M, Rastogi S, Sabbah HN, Stanley
WC. Moderate
severity heart failure does not involve
a downregulation of myocardial fatty
acid oxidation. Am
J Physiol Heart Circ Physiol. 2004 Oct; 287(4):H1538-43.
Epub 2004 Jun 10. PMID: 15191896 [PubMed - indexed for MEDLINE]
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Kerner
J and Bieber
LL. Conversion of carnitine palmitoyltransferase
of rat heart mitochondria into a high affinity,
malonyl-CoA inhibited state. Biochem.Arch., 10:95-101,
1994.
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Kerner
J, Zaluzec
E., Gage D., and Bieber L.L. Characterization of the
malonyl-CoA-sensitive carnitine palmitoyltransferase
(CPTo) of a rat heart mitochondrial particle:
Evidence that the catalytic unit is CPTi. J.Biol.Chem., 269:8209-8219,
1994.
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Zezza F, Kerner
J,
Pascale MR, Giannini R, and Arrigoni-Martelli
E. Rapid
determination of amino acids by high-performance
liquid chromatography: release of amino acids
by perfused liver. J.Chrom., 593:99-101,
1992.
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Lilly K., Chung C., Kerner
J., VanRenterghem R., and Bieber L.L. The effect
of etomoxiryl-CoA on different carnitine
acyltransferases. Biochem.Pharmacol., 43:353-361,
1992.
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DiLisa F., Bieber L.L., Kerner
J., Menabo R., Barbato R., and Siliprandi N. Methods
for carnitine assay. In: L-carnitine and its role in
medicine: from function to therapy, 37-61, 1992. Eds.: Ferrari,
R., DiMauro., and Sherwood, G., Academic
Press.
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Schmidt-Sommerfeld E.,
Penn D., Bieber L.L., Kerner J., Rossi T.M. and Lebenthal
E. Carnitine ester excretion in pediatric patients
receiving parenteral nutrition. Pediatr.Res., 28:158-165,
1990.
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Melegh B., Kerner J.,
Acsadi Gy., Lakatos J., and Sandor A. L-carnitine
replacement therapy in chronic valproate
treatment. Neuropediatrics, 21:40-43,
1990.
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Kerner J., and Bieber
L.L. Isolation of a malonyl-CoA sensitive CPT/ -oxidation
enzyme complex from rat heart mitochondria. Biochemistry, 29:4326-4334,
1990.
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Melegh B, Kerner
J,
Jaszai V, and Bieber LL. Differential excretion
of xenobiotic acyl-esters of carnitine due
to pivampicillin and valproate. Biochem.Med.Metab.Biol., 43:30-38,
1990.
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Schmidt-Sommerfeld E, Penn
D, Kerner J, Bieber LL, Rossi TM, and Lebenthal E. Quantitation
of urinary carnitine esters in a patient
with medium-chain acyl-coenzyme A dehydrogenase
deficiency: effect of metabolic state and
L-carnitine therapy. Pediatr., 115:577-582,
1989.
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Schmidt-Sommerfeld E,
Penn D, Kerner J, and Bieber LL. Analysis
of acylcarnitines in normal human urine with
the radioisotopic exchange-high performance
liquid chromatography (HPLC) method. Clin.Chim. Acta, 181:231-238,
1989.
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Healy MJ, Kerner
J,
and Bieber LL. Enzymes of carnitine acylation. Is
overt carnitine palmitoyltransferase of liver
peroxisomal carnitine octanoyltransferase? Biochem.J., 249:231-237,
1988.
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Melegh B, Szucs L, Kerner
J, and Sandor A. Changes of plasma free
amino acids and renal clearances of carnitines
in premature infants during L-carnitine
supplemented human milk feeding. J. Pediatr. Gastroenterol.
Nutr., 7: 424-429,
1988.
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Fiol CJ, Kerner
J,
and Bieber LL. Effect of malonyl-CoA on the kinetics
and substrate cooperativity of membrane-bound
carnitine palmitoyltransferase of rat heart
mitochondria. Biochem. Biophys. Acta, 916: 482-492,
1987.
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Melegh B, Kerner
J,
and Bieber LL. Pivampicillin-promoted excretion of
pivaloylcarnitine in humans. Biochem. Pharmacol., 36:
3405-3409, 1987.
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Melegh B, Kerner
J,
Kispal Gy, Acsadi Gy, and Dani M. Effect of chronic
valproic treatment on plasma and urine carnitine
levels in children: decreased urinary excretion. Acta
Paed. Hung., 28: 137-142,
1987.
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Melegh B, Kerner
J,
Sandor A, Vinceller M, and Kispal Gy. Effects of
oral L-carnitine supplementation in low-birth-weight
premature infants maintained on human milk. Biol.Neonate, 51:
185-193, 1987.
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Romsos DR, Vander Tuig
JG, Kerner J, and Grogan CK. Energy balance
in rats with obesity-producing hypothalamic
knife cuts: effects of adrenalectomy. J.Nutr., 117:
1121-1128, 1987.
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Bieber LL and Kerner
J. Short-chain acylcarnitines: identification
and quantification. Methods in Enzymology, 123:
H264-276, 1986; Eds. Chytil, F., and McCormick, D.B.,
Academic Press.
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Bieber LL, Lysiak W,
and Kerner J. Use of HPLC for assaying carnitine
derivatives. Clinical aspects of human carnitine
deficiency., Ed. Borum, P.R., Pergamon Press
1986, pp.66-74.
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Melegh B, Kerner
J,
Sandor A, Vinceller M, and Kispal Gy. Oral L-carnitine
supplementation in low-birth-weight newborns:
a study on neonates requiring combined parenteral
and enteral nutrition. Acta
Paed. Hung., 27:253-258, 1986.
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Melegh B, Kerner
J,
Vinceller M, and Kispal Gy. Uptake of beta-hydroxybutyrate,
acetoacetate and glucose by the forearm of
the newborn infant. Acta
Paed. Hung., 27:323-327, 1986.
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Vander Tuig JG, Kerner
J, Crist KA, and Romsos DR. Impaired thermoregulation
in cold-exposed rats with hypothalamic
obesity. Metabolism, 35:960-966,
1986.
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Vander Tuig JG, Kerner
J, and Romsos DR. Hypothalamic obesity, brown
adipose tissue and sympathoadreansl activity
in rats. Amer.J.Physiol., 248:E607-E617,
1985.
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Kerner
J, and Bieber
LL. Isolation and identification of -methyloctanoylcarnitines
from human urine. Prep. Biochem., 15:237-257,
1985.
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Valker K, Ely S, Kerner
J, Scott J, and Bieber LL. Effect of hypoxia
on pig heart short-chain acylcarnitines. Comp.Biochem.Physiol., 80:A123-127,
1985.
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Kerner
J, Froseth
JA, Miller ER, and Bieber LL. A study of the
acylcarnitine content of sow's colostrum,
milk and newborn piglet tissues: demonstration
of high amounts of isovalerylcarnitine in
colostrum and milk. J.Nutr., 114:854-861,
1984.
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Kerner
J and Bieber
LL. The effect of electrical stimulation, fasting
and anesthesia on the carnitine(s) and acylcarnitines
of rat white and red skeletal muscle fibers. Comp.Biochem.
Physiol., 75:B311-316,
1983.
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Kerner
J and Bieber
LL. A radioisotopic-exchange method for quantitation
of short-chain (acid-soluble) acylcarnitines. Anal.Biochem., 134:459-466,
1983.
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Sandor A, Kispal Gy, Kerner
J, and Alkonyi I. Combined effect of ascorbic
acid deficiency and underfeeding on the
hepatic carnitine level in guinea-pigs. Experientia, 39:512-513,
1983.
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Kerner
J, and Bieber
L.L. Effect of clofibrate on rat liver acylcarnitines. Biochem.Med., 28:204-209,
1982.
Sandor A, Kerner
J,
and Alkonyi I. On carnitine content of human breast
milk. Pediatr.Res., 16:89-91, 1982.
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Sandor A, Kerner
J,
and Alkonyi I. Concurrent occurrence of elevated ketone
body and depressed carnitine level in underfed
guinea-pigs. Experientia, 37:933-934,
1981.
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Sandor A, Kerner
J,
and Alkonyi I. Role of carnitine in promoting the
effect of antidiabetic biguanides on hepatic
ketogenesis. Biochem. Pharmacol., 28:969-974,
1979.
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Sandor A, Kerner
J,
and Alkonyi I. Effect of a biguanide (buformin) on
carnitine palmitoyl-transferase activity.
Acta Biochim.Biophys. Acad.
Sci. Hung., 12:217-221, 1977.
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Kerner J,
Sandor A, and Alkonyi I. The effect of denervation
on carnitine metabolism in rat skeletal muscle. Acta Biochim.Biophys. Acad.
Sci. (Hung.), 11:239-243, 1976.
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Alkonyi I, Kerner
J,
and Sandor A. The possible role of carnitine and carnitine
acetyltransferase in the contracting frog
skeletal muscle. FEBS
Letters, 52:265-268, 1975.
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Alkonyi I, Kerner
J,
and Sandor A. The effect of cold and fasting on the
carnitine concentration in the brown adipose
tissue of rats. Acta Biochim.Biophys.
Acad. Sci. (Hung.), 10:217-220, 1975.
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Kerner
J, Sandor,
A., and Alkonyi, I. Physiological variations in carnitine
acetyltransferase activity in rat tissues:
activity in animals exposed to fasting and
cold. Acta Physiol. Acad. Sci. (Hung.), 43:227-231,
1973.
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Alkonyi I, Kerner
J, and Szabo D. A new enzymatic method
for the determination of acetoacetate.
Acta Biochim.Biophys. Acad.
Sci. (Hung.), 7 :143-147, 1972.
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Alkonyi
I, Kerner
J,
Palfi E, and Sandor A. Notiz über die Spaltung
von 5-oxo-3-methyl-2-hexensäure. Chemische Berichte, 104 :1699-1700,
1971.
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PubMed
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