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CA2029
Metabolic Core

Acute portal vein catherization

Acute arterial or jugular vein catherization

Link to mmpc.org shared catalog

 

CA2030 Metabolic Core

Implant
[G2-Mitters™]

Adult mice will be anesthetized and the G2-Mitters™ will be inserted through an incision in the abdominal cavity and anchored with suture to the muscle wall.  Animals will be allowed to recover for at least seven days before the onset of testing.  The device will measure body temperature, activity and heart rate.

Link to mmpc.org shared catalog

 

CA2031 Metabolic Core

Long-term analysis of surgery implantation of Min-Mitter™

 

 

Link to mmpc.org shared catalog

 

CA2043 Analytical Core

 

Portal vein injection

To observe the effects in insulin signaling, insulin (1 milli-unit · kg-1 body weight) will be administered to anesthetize mice by injection into the portal vein. Liver, skeletal muscle and fat biopsies will be removed before basal-time (0) and 5 min after the insulin injection. The tissues will be quick-frozen for future analysis via Western Blot by investigator.

1.    Millward CA, DeSantis D, Hsieh CW, Heaney JD, Pisano S, Olswang Y, Reshef L, Beidelschies M, Puchowicz M and Croniger CM.  Phosphoenolpyruvate carboxykinase (Pck1) in hepatic and adipose tissue contributes to the regulation of the triglyceride fatty acid cycle and development of insulin resistance in mice.  J Lipid Res 51: 1452-63 (2010).   PMID: 20124556  Full Text (PDF)

CA2044 Analytical Core

Brain uptake and blood flow

 

 

CA2046 Analytical Core

Indirect calorimetry

Both total energy expenditure and relative rates of carbohydrate versus fat oxidation will be determined via indirect calorimetry measured by CO2 production in specially-designed chambers for mice.

 

CA2047 Analytical Core

Treadmill training / Endurance Study and IC

Both total energy expenditure and relative rates of carbohydrate versus fat oxidation will be determined via indirect calorimetry measured by CO2 production in specially designed treadmill chambers for mice.

 

CA2045 Analytical Core

ATP/ADP

ATP tissue concentrations will be analyzed enzymatically from 25-30 mg of tissue and reported as nmol/mg wet tissue weight.

1.    Millward CA, DeSantis D, Hsieh CW, Heaney JD, Pisano S, Olswang Y, Reshef L, Beidelschies M, Puchowicz M and Croniger CM.  Phosphoenolpyruvate carboxykinase (Pck1) in hepatic and adipose tissue contributes to the regulation of the triglyceride fatty acid cycle and development of insulin resistance in mice.  J Lipid Res 51: 1452-63 (2010).   PMID: 20124556     Full Text (PDF)

CA2053CB Analytical Core

Custom-designed METABOLIC Experiments

We help users to design and implement in vivo experiments aimed at unraveling the metabolic process they are investigating. Protocols use a combination of physiological, metabolic and, if needed, isotopic techniques. The experiments and/or analyses are conducted in the user's lab or at the MMPC, with some of the analyses conducted in one of the MMPCs.

 

 

See complete list of referenced publication HERE