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Test No.

Test Name

Description

References

CA2000
Metabolic Core

Body Composition

Food consumption is measured over a 24-hour period. This is done once a week for the duration of the study.

Link to mmpc.org  shared catalog

 

CA2001
Metabolic Core

Food Consumption

Food consumption is measured over a 24-hour period. This is done once a week for the duration of the study.

Link to mmpc.org  shared catalog

1.  Berry DC, Noy N.  All-trans-retinoic acid represses obesity and insulin resistance by activating both peroxisome proliferation-activated receptors.   Mol Cell Biol. 2009 Jun; 29(12): 3286-96. Epub 2009 Apr 13.   PMCID: PMC2698724
Full Text (PDF)

CA2002 Metabolic Core

Body Weight

Mice are weighed 1-3 times per week for the duration of the study.

 

Link to mmpc.org shared catalog

1.    Berry DC, Noy N.  All-trans-retinoic acid represses obesity and insulin resistance by activating both peroxisome proliferation-activated receptors.   Mol Cell Biol. 2009 Jun; 29(12): 3286-96. Epub 2009 Apr 13.   PMCID: PMC2698724  Full Text (PDF)

CA2003
Metabolic Core

Continuous measurement of body temperature (by probe)

Body temperature will be measured by anal probe for a short period of time. This can be done at room temperature or under cold challenge conditions.

Link to mmpc.org shared catalog

 

CA2004
Metabolic Core

GTT

Glucose
Tolerance
Test

Mice will be fasted for 5 hours. A fasting blood sample will be removed from the tail vein and a concentrated solution of glucose injected into the abdominal cavity of the mice through a needle passed through the abdominal skin. 

Blood samples (~ 5ul) will be removed from the tail vein 15, 30, 60 and 120 minutes later and glucose will be measured by glucometer.

 

Link to mmpc.org shared catalog

1.    Millward CA, DeSantis D, Hsieh CW, Heaney JD, Pisano S, Olswang Y, Reshef L, Beidelschies M, Puchowicz M and Croniger CM.  Phosphoenolpyruvate carboxykinase (Pck1) in hepatic and adipose tissue contributes to the regulation of the triglyceride fatty acid cycle and development of insulin resistance in mice.   J Lipid Res. 2010 Jun; 51(6): 1452-63. Epub 2010 Feb 2.   PMID: 20124556
Full Text (PDF)

2.    Ayala JE, Samuel VT, Morton GJ, Obici S, Croniger CM, Shulman GI, Wasserman DH, McGuinness OP; NIH Mouse Metabolic Phenotyping Center Consortium. Standard operating procedures for describing and performing metabolic tests of glucose homeostasis in mice.  Dis Model Mech. 3(9-10): 525-34 (2010).     PMCID: PMC2938392 [Available on 2011/9/1]

CA2005 Metabolic Core

Insulin concentrations at fasting and post intraperitoneal glucose administration

Mice will be fasted for 5 hours. A fasting blood sample will be removed from the tail vein and a concentrated solution of glucose injected into the abdominal cavity of the mice through a needle passed through the abdominal skin.  Thirty minutes later, another blood sample will be taken.  Insulin in plasma at time 0 and 30 min. will be measured by ELISA. (CA2006)

link to mmpc.org shared catalog

1.    Millward CA, DeSantis D, Hsieh CW, Heaney JD, Pisano S, Olswang Y, Reshef L, Beidelschies M, Puchowicz M and Croniger CM.  Phosphoenolpyruvate carboxykinase (Pck1) in hepatic and adipose tissue contributes to the regulation of the triglyceride fatty acid cycle and development of insulin resistance in mice.  J Lipid Res 51: 1452-63 (2010).   PMID: 20124556  Full Text (PDF)

CA2006 Metabolic Core

Plasma insulin measurement by ELISA

We will measure insulin concentration in plasma from mice (~25ul/mouse in duplicate) by commercially available ELISA.

 

link to mmpc.org shared catalog

1.    Millward CA, DeSantis D, Hsieh CW, Heaney JD, Pisano S, Olswang Y, Reshef L, Beidelschies M, Puchowicz M and Croniger CM.  Phosphoenolpyruvate carboxykinase (Pck1) in hepatic and adipose tissue contributes to the regulation of the triglyceride fatty acid cycle and development of insulin resistance in mice.  J Lipid Res 51: 1452-63 (2010).   PMID: 20124556  Full Text (PDF)

CA2007 Metabolic Core

Insulin concentrations at fasting and post intraperitoneal insulin administration

Insulin concentrations at fasting and post intraperitoneal insulin
administration.

 

link to mmpc.org shared catalog

 

CA2008 Metabolic Core

I T T

Mice will be fasted for 5 hours and anesthetized.  A fasting blood sample will be removed from the tail vein and insulin (0.5mU/g) will be injected into the abdominal cavity.  Blood samples will be removed from the tail vein 15, 30, 45 and 60 minutes later.  Samples of plasma obtained during the test will be measured for concentrations of glucose and insulin.

Link to mmpc.org shared catalog

1.    Ayala JE, Samuel VT, Morton GJ, Obici S, Croniger CM, Shulman GI, Wasserman DH, McGuinness OP; NIH Mouse Metabolic Phenotyping Center Consortium. Standard operating procedures for describing and performing metabolic tests of glucose homeostasis in mice.  Dis Model Mech. 3(9-10): 525-34 (2010).   PMCID: PMC2938392 [Available on 2011/9/1]  Full Text (PDF)

CA2009 Metabolic Core

Triglycerides in liver

A small piece of liver tissue will be homogenized and liver triglycerides will be saponified in KOH.  Glycerol will be measured against glycerol standards using a commercially available reagent set.

  

Link to mmpc.org shared catalog

1.    Buchner DA, Burrage LC, Hill A, O'Brien WE, Croniger CM, Nadeau JH.  Resistance to diet-induced obesity in mice with a single substituted chromosome.  Physiol. Gen. 35(1): 116-22 (2009).    PMCID: PMC2536825  Full Text (PDF)

2.    Millward CA, Burrage LC, Shao H, Sinasac DS, Kawasoe JH, Hill-Baskin AE, Ernest SR, Gornicka A, Hsieh CH, Pissano S, Nadeau JH and Croniger CM.  Genetic factors for resistance to diet-induced obesity and associated metabolic traits on mouse chromosome 17.   Mamm Genome. 2009 Feb; 20(2): 71-82. Epub 2009 Jan 10.  PMID: 19137372
Full Text (PDF)

CA2010 Metabolic Core

Plasma Triglycerides

Plasma triglycerides will be saponified in KOH. Glycerol will be measured against glycerol standards using a commercially available reagent set.

Link to mmpc.org shared catalog

1.    Millward CA, Burrage LC, Shao H, Sinasac DS, Kawasoe JH, Hill-Baskin AE, Ernest SR, Gornicka A, Hsieh CH, Pissano S, Nadeau JH and Croniger CM.  Genetic factors for resistance to diet-induced obesity and associated metabolic traits on mouse chromosome 17.   Mamm Genome. 2009 Feb; 20(2): 71-82. Epub 2009 Jan 10.  PMID: 19137372
Full Text (PDF)

CA2011 Analytical Core

Total Energy Expenditure using: 2H-labeled water

 

Link to mmpc.org shared catalog

 

CA2013 Metabolic Core

Hyperinsulinemic clamp

The euglycemic-hyperinsulinemic clamp is used to investigate insulin action and glucose metabolism in the intact, conscious mouse. The use of different glucose or glucose analog tracers with the option of using dual tracers (3-H or 14-C labeled) allows the determination of tissue-specific glucose uptake in addition to whole body glucose production and disposal. We can also use stable isotopes for the glucose clamp studies.

A chronic in-dwelling catheter is surgically implanted into the right jugular vein of the mouse.  After a minimum of 4 days of recovery, the mice are fasted the morning of the experiment.  The 120-minute protocol begins with a prime-continuous infusion of glucose tracer and a prime-continuous infusion of insulin, at a rate dependant on the goals and conditions of the experiment.  Glucose is infused at a rate that maintains euglycemia and is a measure of insulin sensitivity.  Tissues can be collected at the conclusion of the clamp for later assessment of tissue-specific glucose uptake as well as other biochemical assays as desired.

1.    Ayala JE, Samuel VT, Morton GJ, Obici S, Croniger CM, Shulman GI, Wasserman DH, McGuinness OP; NIH Mouse Metabolic Phenotyping Center Consortium. Standard operating procedures for describing and performing metabolic tests of glucose homeostasis in mice.   Dis Model Mech. 2010 Sep-Oct; 3(9-10): 525-34. Epub 2010 Aug 16.  PMCID: PMC2938392 [Available on 2011/9/1]

CA2023

 

 

 

CA2024
Metabolic Core

Metabolomic profile of citric acid cycle and gluconeogenic intermediates

 

Link to mmpc.org shared catalog

 

CA2024CT
Metabolic Core

Custom-designed TRACER Experiments

We help users design experiments aimed at measuring:   (i) carbon flux through a pathway, or  (ii) the distribution of carbon fluxes through multiple pathways.

We emphasize the need to distinguish net carbon flux from isotopic flux. The latter often results from net carbon flux, isotopic exchange, or both. We suggest the use of isotopic substrate(s) and analytical equipment (mass spectrometry, NMR) most appropriate to the problem being studied. If the isotopic substrate is not commercially available, we can, in some cases, arrange for a pilot synthesis of the compound in an academic lab.

We welcome users and their staff to spend time in our lab to learn and practice isotopic techniques.

 

CA2025
Metabolic Core

Chronic arterial and jugular vein catherization

Chronic arterial and jugular vein catherization

Link to mmpc.org shared catalog

 

CA2026
Metabolic Core

Chronic arterial
or jugular vein catherization

Chronic arterial or jugular vein catherization

Link to mmpc.org shared catalog

 

CA2027
Metabolic Core

Acute arterial
and jugular vein catherization

Acute arterial and jugular vein catherization

Link to mmpc.org shared catalog

 

CA2028
Metabolic Core

Acute arterial
or jugular vein catherization

Acute arterial or jugular vein catherization

Link to mmpc.org shared catalog

 

CA2029
Metabolic Core

Acute portal vein catherization

Acute arterial or jugular vein catherization

Link to mmpc.org shared catalog

 

CA2030 Metabolic Core

Implant
[G2-Mitters™]

Adult mice will be anesthetized and the G2-Mitters™ will be inserted through an incision in the abdominal cavity and anchored with suture to the muscle wall.  Animals will be allowed to recover for at least seven days before the onset of testing.  The device will measure body temperature, activity and heart rate.

Link to mmpc.org shared catalog

 

CA2031 Metabolic Core

Long-term analysis of surgery implantation of Min-Mitter™

 

 

Link to mmpc.org shared catalog

 

CA2043 Analytical Core

 

Portal vein injection

To observe the effects in insulin signaling, insulin (1 milli-unit · kg-1 body weight) will be administered to anesthetize mice by injection into the portal vein. Liver, skeletal muscle and fat biopsies will be removed before basal-time (0) and 5 min after the insulin injection. The tissues will be quick-frozen for future analysis via Western Blot by investigator.

1.    Millward CA, DeSantis D, Hsieh CW, Heaney JD, Pisano S, Olswang Y, Reshef L, Beidelschies M, Puchowicz M and Croniger CM.  Phosphoenolpyruvate carboxykinase (Pck1) in hepatic and adipose tissue contributes to the regulation of the triglyceride fatty acid cycle and development of insulin resistance in mice.  J Lipid Res 51: 1452-63 (2010).   PMID: 20124556  Full Text (PDF)

CA2044 Analytical Core

Brain uptake and blood flow

 

 

CA2046 Analytical Core

Indirect calorimetry

Both total energy expenditure and relative rates of carbohydrate versus fat oxidation will be determined via indirect calorimetry measured by CO2 production in specially-designed chambers for mice.

 

CA2047 Analytical Core

Treadmill training / Endurance Study and IC

Both total energy expenditure and relative rates of carbohydrate versus fat oxidation will be determined via indirect calorimetry measured by CO2 production in specially designed treadmill chambers for mice.

 

CA2045 Analytical Core

ATP/ADP

ATP tissue concentrations will be analyzed enzymatically from 25-30 mg of tissue and reported as nmol/mg wet tissue weight.

1.    Millward CA, DeSantis D, Hsieh CW, Heaney JD, Pisano S, Olswang Y, Reshef L, Beidelschies M, Puchowicz M and Croniger CM.  Phosphoenolpyruvate carboxykinase (Pck1) in hepatic and adipose tissue contributes to the regulation of the triglyceride fatty acid cycle and development of insulin resistance in mice.  J Lipid Res 51: 1452-63 (2010).   PMID: 20124556     Full Text (PDF)

CA2053CB Analytical Core

Custom-designed METABOLIC Experiments

We help users to design and implement in vivo experiments aimed at unraveling the metabolic process they are investigating. Protocols use a combination of physiological, metabolic and, if needed, isotopic techniques. The experiments and/or analyses are conducted in the user's lab or at the MMPC, with some of the analyses conducted in one of the MMPCs.

 

 

See complete list of referenced publication HERE