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The CASE MMPC specializes on metabolic investigations with stable isotopes and on the training of investigators who are studying mouse models of metabolic diseases.
The Center uses mass spectrometry and labeling patterns to calculate metabolic fluxes such as: LIPID, PROTEIN and GLUCOSE turnover. The Center uses specialized, and in some cases, unique isotopic techniques to investigate the regulation of metabolic pathways and their intracellular compartmentation.
We conduct studies and analyses on mice or mice samples shipped to our facility. We are available to discuss with potential users the design and interpretation of experiments to be conducted in their labs. We also train their staff in analytical techniques. We can also set up new analyses of unlabeled and labeled metabolites.
If re-derivation of mice shipped to our center is required, the CASE Transgenic and Targeting Core (Dr. Ron Conlon, Director) can provide this service. 'Imaging of Mice Organs' can also be conducted by the CASE Center for Imaging Research (Dr. Chris Flask & Dr. Jeffrey Duerk).
The Metabolic Core conducts in vivo and ex vivo metabolic experiments on mice and rats. This core provides the following techniques and measurements:
Dr. Croniger discusses with the users the terms of the service contract, helps them get approval for their animal protocol with the Case IACUC, and plans for the shipment of mice to our center. She interacts closely with the Animal Care Core for the testing, quarantine, and housing of all mice.
The Analytical and Metabolomic Core conducts the following types of analyses on tissues, blood, plasma and urine from experiments conducted at the MMPC or at the user's lab:
Dr. Puchowicz and her team work out the terms of the contract, help the users understand the nature of the assays, coordinate the planning studies done off-site and the collection and shipment of samples for analyses. The Analytic Core has a close interaction with the Metabolic Core to perform measurements of glucose, glycerol and/or amino acid turnover that are done in catheterized mice under basal and/or glucose-insulin clamp conditions.
The Animal Care Core is directed by Dr. W. John Durfee and managed by the Animal Resource Center (ARC) which implements the guidelines and regulations associated with all animal research at Case.
ANIMAL CARE CORE DIRECTOR
W. John Durfee, DVM, DACLAM
Joan Schenkel, M.S.
Tel: (216) 368-4268
The CASE MMPC
Case Western Reserve University
10900 Euclid Ave., BRB 9th Floor
Cleveland, OH 44106-4988