Case Western Reserve University Mouse Metabolic Phenotyping Center
Dr. Henri Brunengraber, Dr. Colleen Croniger and Dr. Michelle Puchowicz concentrates on metabolic investigations and on the training of investigators who are studying mouse models of metabolic diseases.  The center uses mass spectrometry or NMR of compounds labeled with stable isotopes.  This allows calculating metabolic fluxes such as lipid, protein, and glucose turnover.  The metabolic and metabolomic groups provide specialized, and in some cases, unique isotopic techniques to investigate the regulation of metabolic pathways and their intracellular compartmentation.  We conduct studies and analyses on mice shipped to CASE.  We also conduct analyses on samples from mice shipped from the user's lab.  We are available to discuss with potential users the design of experiments with isotopic compounds to be conducted in their labs.  We can also set up new analyses of (un)labeled metabolites. If re-derivation of mice shipped to our Center is required, the Case Transgenic and Targeting Core, directed by Dr. Ron Conlon, can provide this service.   Also, imaging of mice organs can be conducted by the Small Animal Imaging facility of the Case Center for Imaging Research (Drs. Chris FLASK and Jeffrey DUERK).