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Welcome to the CASE MMPC |
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CENTER DIRECTOR
Henri BRUNENGRABER, MD, PhD
(216) 368-6548
(216) 368-6560 FAX
henri.brunengraber@case.edu
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Administrative Manager
Laila Boesinger
(216) 368-8821
(216) 368-6644 FAX
laila.boesinger@case.edu |
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| MMPC@case.edu |
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Case Western Reserve University Mouse Metabolic Phenotyping Center
Dr. Henri Brunengraber, Dr. Colleen Croniger and Dr. Michelle Puchowicz concentrates on metabolic investigations and on the training of investigators who are studying mouse models of metabolic diseases. The center uses mass spectrometry or NMR of compounds labeled with stable isotopes. This allows calculating metabolic fluxes such as lipid, protein, and glucose turnover. The metabolic and metabolomic groups provide specialized, and in some cases, unique isotopic techniques to investigate the regulation of metabolic pathways and their intracellular compartmentation. We conduct studies and analyses on mice shipped to CASE. We also conduct analyses on samples from mice shipped from the user's lab. We are available to discuss with potential users the design of experiments with isotopic compounds to be conducted in their labs. We can also set up new analyses of (un)labeled metabolites. If re-derivation of mice shipped to our Center is required, the Case Transgenic and Targeting Core, directed by Dr. Ron Conlon, can provide this service. Also, imaging of mice organs can be conducted by the Small Animal Imaging facility of the Case Center for Imaging Research (Drs. Chris FLASK and Jeffrey DUERK). |
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Colleen Croniger |
(216) 368-4967 |
colleen.croniger@case.edu |
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Henri Brunengraber |
(216) 368-6548 |
henri.brunengraber@case.edu |
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The Metabolic Core conducts in vivo and ex vivo metabolic experiments on mice shipped to the Case MMPC. This core focuses on the following techniques and measurements:
- Chronic or acute arterial, jugular and/or gastric catheterization
- Acute catheterization of portal vein or urinary bladder
- Energy expenditure integrated over four days by the "doubly-labeled water" method
- Rates of fatty acid, cholesterol, triglycerides, or protein synthesis measured using 2H2O
- Insulin clamp, pancreatic clamp
- Food intake
- Body temperature
- Urine and blood chemistry analysis
- Glycosylated hemoglobin
- Liver perfusion and heart perfusion
- Tissue perfusion and fixation
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Michelle Puchowicz |
(216) 368-2501 |
michelle.puchowicz@case.edu |
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Henri Brunengraber
Xin Yu |
(216) 368-6548
(216) 368-3918 |
henri.brunengraber@case.edu
xin.xu@case.edu |
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Tissues, blood, plasma and urine from experiments conducted at the MMPC or at the user's lab are analyzed for:
- 2H- and 18O-enrichments of plasma or urine for total energy expenditure
- 2H-enrichment of plasma or urine for calculating total body water
- turnover of glucose and/or glycerol with [6,6–2H2]glucose and [2H5]glycerol
- Concentration and/or labeling pattern of plasma fatty acids (C8-C22)
- Concentration and/or labeling pattern of plasma aminoacids
- Concentration and/or labeling pattern of acylcarnitines in plasma or urine
- Concentration and/or labeling pattern of long-chain acyl-CoAs in tissues
- Concentration and/or labeling pattern of acetyl-CoA, propionyl-CoA, succinyl-CoA, and methylmalonyl-CoA in tissues
- Concentration and/or labeling pattern of citric acid cycle and gluconeogenic intermediates in tissues
- 13C-labeling pattern of the acetyl moiety of citrate
- activity of acetyl-CoA carboxylase or malonyl-CoA decarboxylase in tissues
- rates of FA and cholesterol synthesis in tissues from the incorporation of 2H or 13C
- rate of protein synthesis from the incorporation of 2H-enriched water
- metabolomic profile in plasma, urine or tissue
- Profile of 120 organic acids in urine
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