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THE ANALYTICAL AND METABOLOMIC CORE

 


CORE DIRECTOR
Michelle PUCHOWICZ, PhD
(216) 368-2501 Direct
(216) 368-6560 FAX
michelle.puchowicz@case.edu

CO-DIRECTORS
Henri BRUNENGRABER
Xin YU
 
MMPC@case.edu



The Analytical Core conducts all assays involving the determination of isotope labeling and concentration profiles
of metabolites including:


          (i)    2H-labeling of water, lipids and amino acids

          (ii)    the 18O-labeling of water

          (iii)   the concentration profile of acyl-CoAs (e.g. short, medium and long-chain species)

          (iv)   metabolic profiles of citric acid cycle intermediates, amino acids and fatty acids


Dr. Puchowicz and her team work out the terms of the contract, help the users understand the nature of the assays, coordinate the planning studies done off-site and the collection and shipment of samples for analyses. The Analytic Core has a close interaction with the Metabolic Core to perform measurements of glucose, glycerol and/or amino acid turnover that are done in catheterized mice under basal and/or glucose-insulin clamp conditions.to prepare the scientific reports to users and the billing for the services.
 

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ORDER TESTS      
 

Tissues, blood, plasma and urine from experiments conducted at the MMPC or at the user's lab are analyzed for:
  • Concentration and/or labeling pattern of acetyl-CoA, propionyl-CoA, succinyl-CoA, and methylmalonyl-CoA in tissues