Dr. Silverman has investigated molecular pathways of antiviral innate immunity for the past 34 years. His studies are mainly focused on the 2’,5’-oligoadenylate (2-5A) synthetase (OAS)/RNase L system, a classical interferon-regulated, innate immune pathway that rapidly responds to PAMPs to produce a broadly active antiviral response. In prior studies, his lab purified and cloned RNase L, generated RNase L-deficient mice, established antiviral and apoptotic activities of RNase L in vivo, and determined that RNase L activation induces autophagy and initiates transcriptional signaling pathways. In current studies, he is investigating control of viral pathogenesis by regulating 2-5A turnover. His goal is to continue probing fundamental events and biologic endpoints that impact on viral replication cycles and cancer biology.
Silverman Lab Webpage
Zhou, A., Hassel, B.A., and Silverman, R.H. Expression cloning of 2-5A-dependent RNase-a uniquely regulated mediator of interferon action. Cell, 72, 753-765, 1993.
Der, S., Zhou, A., Williams, B.R.G., and Silverman, R.H.Identification of Genes Differentially Regulated by IFN-α β or γ or Using Oligonucleotide Arrays. Proc. Natl. Acad. Sci. U.S.A 95,15623-15628, 1998.
Malathi, K., Dong, B., Gale, M., and Silverman, R.H. Small Self RNA Generated by RNase L Amplifies Antiviral Innate Immunity. Nature, 448: 816-819, 2007. [PubMed]
Chakrabarti, A., Ghosh, P.K., Banerjee, S., Gaughan, C., and Silverman, R.H. RNase L triggers autophagy in response to viral infections. J. Virol. 86: 11311-21, 2012. [PubMed]
Zhang, R., Jha, B.K., Ogden, K., Dong, B., Zhao, L., Elliot, R., Patton, J.T., Silverman, R.H.* and Weiss, S.R.* Homologous 2',5'-phosphodiesterases from disparate RNA viruses antagonize antiviral innate immunity. Proc. Natl. Acad. Sci., Epub, 2013. [*Co-corresponding authors]. [PubMed]