My project involves delineating the biochemical mechanism(s) by which P-TEFb is mobilized from the 7SK snRNP nuclear complex upon T cell receptor activation of CD4+ T lymphocytes. I'm also interested in understanding how, after its mobilization, P-TEFb becomes recruited to the HIV LTR to facilitate processive transcription of the latent provirus.
Mbonye U, Karn J. Control of HIV latency by epigenetic and non-epigenetic mechanisms. Curr HIV Res. 2011 Dec 30 Epub ahead of print.
Kim YK, Mbonye U, Hokello J, Karn J. T-cell receptor signaling enhances transcriptional elongation from latent HIV proviruses by activating P-TEFb through an ERK-dependent pathway. J Mol Biol. 2011 Jul 29. Epub 2011 July 14.
Kang YJ, Mbonye UR, DeLong CJ, Wada M, Smith WL. Regulation of intracellular cyclooxygenase levels by gene transcription and protein degradation. Prog Lipid Res. 2007 Mar;46(2):108-25. Epub 2007 Jan 18. [PubMed]
Mbonye UR, Wada M, Rieke CJ, Tang HY, Dewitt DL, Smith WL: The 19-amino acid cassette of cyclooxygenase-2 mediates entry of the protein into the endoplasmic reticulum-associated degradation system. J Biol Chem. 2006 Nov 24;281(47):35770-8. Epub 2006 Sep 25. [PubMed]