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Case Western Reserve University

MOLECULAR BIOLOGY
and MICROBIOLOGY

 
 
       
 

 

Uri Mbonye


Senior Research Associate

Works in: Karn Lab

email: urm2@case.edu

Education:
Eastern Connecticut State University, BSc (Summa Cum Laude) Biology, 2001
Michigan State University, PhD, Biochemistry and Molecular Biology, 2007
Postdoctoral Scholar, Case Western Reserve University, 2007-2010


   
  Research:

We seek to understand how processive transcription of proviral HIV is achieved in latently infected CD4+ T cells, which are well established to be the major reservoir of latent and replication-competent proviruses in HIV+ individuals under life-long antiretroviral therapy. More specifically, my research focuses on studying host and viral control of the processivity transcription factor P-TEFb. This research is being carried out with the understanding that since P-TEFb kinase is absolutely required for proviral transcription, delineating its activation mechanism by cellular signal transduction and HIV Tat, and also the means by which P-TEFb mediates Tat-dependent proviral transcription elongation is of paramount importance in contributing to efforts currently aimed at eradicating latent HIV reservoirs which are resistant to antiretroviral therapy.

 

Select Publications:

Mbonye U., Karn J. Transcriptional control of HIV latency: Cellular signaling pathways, epigenetics, happenstance and the hope for a cure. Virology. 2014 Apr;454-455C:328-339. doi: 10.1016/j.virol.2014.02.008. Epub 2014 Feb 22. Review. [PubMed]

Mbonye U., Gokulrangan G., Datt M., Dobrowolski C., Cooper M., Chance M., Karn J. Phosphorylation of CDK9 at Ser175 enhances HIV transcription and is a marker of activated P-TEFb  in CD4+ T lymphocytes. PLoS Pathogens 2013; 9(5):e1003338. [PubMed]

Mbonye U, Karn J. Control of HIV latency by epigenetic and non-epigenetic mechanisms. Curr HIV Res. (2011) Curr HIV Res, 9(8):554-67. [PubMed]

Kim YK, Mbonye U, Hokello J, Karn J. T-cell receptor signaling enhances transcriptional elongation from latent HIV proviruses by activating P-TEFb through an ERK-dependent pathway. J Mol Biol. 2011 Jul 29. Epub 2011 July 14. [PubMed]

Mbonye UR, Song I. Posttranscriptional and posttranslational determinants of cyclooxygenase expression. BMB Rep. 2009; 42(9):552-60. [PubMed]

Mbonye UR, Yuan C, Harris CE, Sidhu RS, Song I, Arakawa T, Smith WL. Two distinct pathways for cyclooxygenase-2 protein degradation. J Biol Chem. 2008; 283(13):8611-23. [PubMed]

Kang YJ, Mbonye UR, DeLong CJ, Wada M, Smith WL. Regulation of intracellular cyclooxygenase levels by gene transcription and protein degradation. Prog Lipid Res. 2007 Mar;46(2):108-25. Epub 2007 Jan 18. [PubMed]

Mbonye UR, Wada M, Rieke CJ, Tang HY, Dewitt DL, Smith WL: The 19-amino acid cassette of cyclooxygenase-2 mediates entry of the protein into the endoplasmic reticulum-associated degradation system. J Biol Chem. 2006 Nov 24;281(47):35770-8. Epub 2006 Sep 25. [PubMed]