| |
We are studying the role of RNA polymerase phosphorylation and NF-κB in HIV transcription from latent proviruses. Recently we found that NF-κB, in particular the p65 subunit, is able to stimulate both transcription elongation and initiation from the HIV LTR in the transfection system. In order to extend our study in latent proviruses, we established a model system in which populations of Jurkat T cells are infected with HIV vectors carrying fluorescent protein reporter genes. Using high resolution chromatin immunoprecipitation (ChIP) assays we examine the recruitment and modification of RNA polymerase and other transcription factors mediated by NF-κB during proviral reactivation. Although NF-κB is a primary trigger used to reactivate latent proviruses, it remains unclear to what extent other enhancer proteins contribute to these events. We are also studying how cellular signals or enhancer proteins are used to regulate both the magnitude and the duration of HIV transcription.
Selected Publications
Young Kyeung Kim, Ensung Junn, Inwon Park, Younghoon Lee, Changwon Kang, and Jeong Keun Ahn (1999) Repression of hepatitis B virus X gene expression by hammerhead ribozymes, Biochem. Biophys. Res. Commun. 21;257(3):759-65. [PubMed]
Cyril F Bourgeois, Young Kyeung Kim, Mark J. Churcher, Michelle West, and Jonathan Karn (2002) Spt5 cooperates with human immunodeficiency virus type 1 Tat by preventing premature RNA release at terminator sequences. Mol. Cell. Biol. 22(4):1079-93. [PubMed]
Young Kyeung Kim, Cyril F Bourgeois, Catherine Isel, Mark J. Churcher, and Jonathan Karn (2002)Phosphorylation of the RNA polymerase II carboxyl-terminal domain by CDK9 is directly responsible for human immunodeficiency virus type 1 Tat-activated transcriptional elongation.Mol. Cell. Biol. 22(13):4622-37. [PubMed]
Jeong-Ki Kim, Young Kyeung Kim, Junbae Hong, Sang Yong Kim, Chong-Kil Lee, Chul Joong Kim, Young Sang Kim, and Jeong Keun Ahn (2003) Isolation of the Enhanced neurovirulent HSV-1 strains from Korean Patients. Virus Genes 26 (2):115-118. [PubMed]
|
