Postdoctoral Research Associate
Department of Orthopaedics
Case Western Reserve University
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Research Interests
The future of pharmaceutical treatment lies in the production
of targeted delivery systems. With this in mind I am involved
in several research projects which aim to target adult
stem cells to pathological tissues. This work is many
faceted in its development, we are investigating two methods
of targeting the cells: Antibody Directed Cell Therapy
and Peptide Directed Cell Therapy.
With Antibody Directed Cell Therapy we first coat the
cells with palmitated protein G, this is a modified recombinant
peptide that binds to the Fc region of many antibodies.
This provides a general method to develop optimal coating
of antibodies for attachment to many different disease
or injury states. This is primarily being investigated
for attachment and repair of cartilage. In collaboration
with Lauren Buerkle we are investigating the lipid anchor
for its efficiency of integration and the strength of
the interaction once it is in the membrane.
Peptide Directed Cell Therapy is being investigated in
two injury models: Ischemic heart and Ischemic limb. Phage
display has produced peptides which attach to neovascular
tissue. It is thought that these will be able to attach
in the two injury models. These peptides have been labelled
with a fluorophore and injected into the models and localisation
studied by whole body and tissue sectioning.
CV
Education
University of Strathclyde, UK - Biochemistry & Pharmacology
- The effect of hypoxia on lung endothelial nitric oxide
formation - B.Sc. 2000
University of Strathclyde, UK - Pharmaceutical Analysis
- Production of a general method for the detection of
hydrazine in pharmaceuticals - M.Sc. 2002
University of Cardiff, UK - Development of a Synthetic
uPAR Targeted Gene Delivery System - Pharmacy PhD 2006
Publications
1) T. Kean, M. Thanou, Invited book chapter on Chitin
and Chitosan in Polymeric Biomaterials - In Press
2) T. Kean, J. H. McB. Miller, G.G. Skellern, D. Snodin,
Acceptance Criteria for Levels of Hydrazine in Substances
for Pharmaceutical Use and Analytical Methods for its
Determination, Pharmeuropa Scientific Notes, (2006) Abstract
3) T. Kean, Development of a synthetic uPAR targeted
gene delivery system (2006) PhD Thesis, Cardiff University
4) T. Kean, S. Roth, M. Thanou, Trimethylated chitosans
as non-viral gene delivery vectors: Cytotoxicity and transfection
efficiency. J. Control. Release, 103(3) (2005) 643-53
Abstract
5) T. Kean, S. Roth, M. Thanou, Trimethylation of chitosans:
Its effect on toxicity and their application as a non-viral
gene delivery system. In Journal of Pharmacy and Pharmacology,
BPC Science abstracts (2004), Poster Session 2, #128 Abstract
6) T.J. Kean, C.A. Jansma, G. Borchard, M. Thanou. Targeting
The uPA Receptor Using Polymer-Ligand Conjugates For Non
Viral Gene Delivery. in Proc. Int. Symp. Control. Release
Bioact. Mater. 2003. Glasgow, UK
Presentations
1) Development of a synthetic uPAR targeted gene delivery
system - Invited lecture at NMP in Lecce, Italy, 2005
2) Targeted gene delivery for the treatment of cancer
- at Speaking of Science, Cardiff, UK, 2005
3) Trimethylation of chitosans: Its effect on toxicity
and their application as a non-viral gene delivery system
- at The British Pharmaceutical Conference, Manchester,
UK, 2004
4) Effect of the Degree of Chitosan Quaternisation on
Cytotoxicity - at 5th International Symposium for Polymer
Therapeutics, Cardiff, UK, 2005
5) Targeting Therapies to Tumours - at Speaking of Science,
Cardiff, UK, 2004
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