Assistant Professor
Department of Orthopaedics
Case Western Reserve University
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Research Interests
See the Research Foci!!
CV
EDUCATION
Doctor of Philosophy, Biology, Case Western Reserve University,
Cleveland, OH, 1995;
Advisor: Arnold I. Caplan, Ph.D.; Thesis: Mesenchymal
Progenitor Cells in Adult Marrow.
Post-Graduate Training, Electron Microscopy, Hofstra
University, Hempstead, NY, 1976-1978
Bachelor of Science, Biology, State University of New
York, Brockport, NY, 1975.
ACADEMIC HONORS AND AWARDS
" Young Investigator Award, 1995, Fifth International
Conference on the Chemistry and Biology of Mineralized
Tissues, Kohler, WI.
" Young Investigator Award, 1998, Sixth International
Conference on the Chemistry and Biology of Mineralized
Tissues, Vittel, France.
REFEREED PUBLICATIONS
Weidenbecher M, Tucker HM, Awadallah A, Dennis JE (2008)
Fabrication of a Neotrachea Using Engineered Cartilage.
Laryngoscope Jan 14 [Epub ahead of print] Abstract
Dennis JE, Esterly K, Awadallah A, Parrish CR, Poynter
GM, Goltry KL (2007) Clinical-scale expansion of a mixed
population of bone-marrow-derived stem and progenitor
cells for potential use in bone-tissue regeneration. Stem
Cells 25(10):2575-82 Abstract
Chen X, Song IH, Dennis JE, Greenfield EM (2007) Endogenous
PKI gamma limits the duration of the anti-apoptotic effects
of PTH and beta-adrenergic agonists in osteoblasts. J
Bone Miner Res. 22(5):656-64 Abstract
Love, Z., Wang, F, Dennis, J, Awadallah, A, Salem, N,
Lin, Y, Weisenberger, A., Majewski, S., Gerson, S, Lee,
Z (2007) Imaging of mesenchymal stem cell transplant by
bioluminescence and PET. J Nucl Med 48:2011-2020. Abstract
Caplan, A. I. and Dennis, J. E. (2006) Mesenchymal stem
cells as trophic mediators. J Cellular Biochem 98:1076-1084.
Abstract
Bing, M, Hankenson, K.D., Dennis, J.E., Caplan, A.I.,
Goldstein, S.A., Kilbourne, M.R. (2005) A simple method
for stem cell labeling with fluorine 18. Nucl. Med. Biol.
32:701-705. Abstract
Nishioka, K., Dennis, J.E., Gao, J., Goldberg, V. M.,
Caplan, A.I. (2004) Sustained Wnt protein expression in
chondral constructs from mesenchymal stem cells. J. Cellular
Physiol. 203:6-14. Abstract
Naumann, A., Dennis, J.E., Aigner, J., Coticchia, J.,
Arnold, J., Berghaus A., Kastenbauer, E.R., Caplan, A.I.
Tissue Engineering Of Autologous Cartilage Grafts in Three
Dimensional In Vitro Macro Aggregate Culture System. Tiss.
Eng. 10: 1695-1706. Abstract
Dennis, J.E., Caplan, A.I. (2004) Advances in mesenchymal
stem cell biology. Curr. Op. Orthop. 15:341-346. Abstract
Dennis, J.E., Cohen, N., Caplan, A. I., Goldberg, V.M.
(2004) Targeted Delivery of Progenitor Cells for Cartilage
Repair. J. Orthop. Res. 22:735-741. Abstract
Maitra, B., Szekely, E., Gjini, K., Laughlin, M.J., Dennis,
J., Haynesworth, S. E., Koç, O.N. (2004) Human
mesenchymal stem cells support unrelated donor hematopoietic
stem cells and suppress T-cell activation. Bone Marrow
Transpl. 33:597-604. Abstract
Tallheden, T., Dennis, J. E., Lennon, D. P., Sjögren-Jansson,
E., Caplan, A.I., Lindahl, A. (2003) Phenotypic plasticity
of human articular chondrocytes. J. Bone Joint Surg. 85-A(Suppl.):93-99.
Abstract
Yamashita, K., Dennis, J.E., Lennon, D. P., Norimoto,
H., Kitamura, S., Caplan, A. I. (2003) Dental pulp cells
with multi-potential for differentiation to odontoblast
and chondroblast. J. Hard Tiss. Biol. 12:49-55.
Dennis, J.E., Caplan, A.I. (2003) Bone marrow mesenchymal
stem cells. In: "Stem Cell Handbook", Humana
Press, Inc., Totowa, N.J., Sell, S. (Ed.), pp. 107-117.
Dennis, J.E., Sochaga, L., Caplan, A.I. (2003) "Human
Mesenchymal Stem Cells for Cartilage Repair., In: "Mesenchymal
Stem Cells: Biology and Potential Clinical Uses",
Ministerio De Sanidad Y Consumo, Madrid, Grisolía,
S, Miñana, M.D., Bendala Tufanisco, E. (Eds.),
p.p.157-168.
Naumann, A., Dennis, J., Studenmaier, R, Totter, N., Aigner,
J., Ziegelaar, B., Happ, T., Rasp, G., Caplan, A. I. (2002)
Adulte mesenchymale stammzellen neue möglichkeiten
der gewebezüchtung fur die plastich rekonstruktive
chururgie. Laryngo Rhino Otologie 81:521-527. Abstract
Naumann, A., Dennis, J. E., Awadallah, A., Carrino, D.
A., Mansour, J. M., Kastenbauer, E., Caplan, A I. (2002)
Immunochemical and mechanical characterization of cartilage
subtypes in rabbit. J. Histochem. Cytochem. 50:1049-1058.
Abstract
Dennis, J. E., Charbord, P. (2002) Origin and differentiation
of human and murine stroma. Stem Cells 20:205-214. Abstract
Gao, J., Dennis, J. E., Solchaga, L.A., Goldberg, V. M.,
Caplan, A. I. (2002) Repair of osteochondral defect with
tissue engineered two phase composite material of injectable
calcium phosphate and hyaluronan sponge. Tissue Eng 8:827-837.
Abstract
Solchaga, L. A., Gao, J., Dennis, J. E., Awadallah, A.,
Lundberg, M., Caplan, A. I., Goldberg, V. M. (2002) Treatment
of osteochondral defects with autologous bone marrow in
a hyaluronan based delivery vehicle. Tissue Eng 8:333-347.
Abstract
Dennis, J. E., Solchaga, L. A., Caplan, A. I. (2001) Mesenchymal
stem cells for musculoskeletal tissue engineering. New
Surg. 1:112-115.
Dennis, J. E., Carbillet, J. P., Caplan, A. I., Charbord,
P. (2001) The STRO-1+ Marrow Cell Population Is Multipotential.
Cells Tiss. Organs 170:73-82. Abstract
Gao, J., Dennis, J. E., Muzic, R. F., Lundberg, M., Caplan,
A. I. (2001) The dynamic in vivo distribution of bone
marrow derived mesenchymal stem cells after infusion.
Cells Tiss. Organs 169:12-20. Abstract
Dennis, J. E., Carrino, D. A., Yamashita, K., Caplan,
A. I. (2000) Monoclonal antibodies to mineralized matrix
molecules of the avian eggshell. Matrix Biol. 19:683-692.
Abstract
Solchaga, L. A., Yoo, J. U., Lundberg, J., Dennis, J.
E., Huibregtse, B. A., Goldberg, V. M., Caplan, A. I.
(2000) Hyaluronan based polymers in the treatment of osteochondral
defects. J. Orthop. Res. 18:773-780. Abstract
Caplan, A. I., Dennis, J. E. (2000) Genetically linked
scientists: The one two punch for NFATp knockout. J. Exp.
Med. 191:1-3. Abstract
Remy Martin, J. P., Marandin, A., Challier, B., Bernard,
G., Deschaseaux, M., Herve, P., Wei, Y., Tsuji, T., Auerbach,
R., Dennis, J. E., Moore, K. A., Greenberger, J. S., Charbord,
P. (1999) The vascular smooth muscle differentiation of
murine stroma. A sequential model. Exp. Hematol. 27:1782-1795.
Abstract
Solchaga, L. A., Yoo, J. U., Lundberg, J., Dennis, J.
E., Huibregtse, B. A., Goldberg, V. M., Caplan, A. I.
(1999) Hyaluronic acid-based polymers as cell carriers
for tissue-engineered repair of bone and cartilage. J.
Orthop. Res. 17:205-213. Abstract
Dennis, J. E., Merriam, A., Awadallah, A., Yoo, J. U.,
Johnstone, B., Caplan, A. I. (1999) A quadripotential
mesenchymal progenitor cell isolated from the marrow of
an adult mouse. J. Bone Min. Res. 14:700-709. Abstract
Dennis, J. E., Konstantakos, M., Arm, D., Caplan, A. I.
(1998) In vivo osteogenesis assay: A rapid method for
quantitative analysis. Biomat. 19:1323-1328. Abstract
Allay, J. A., Dennis, J. E., Haynesworth, S. E., Majumdar,
M. K., Clapp, D. W., Shultz, L. D., Caplan, A. I., Gerson,
S. L. (1997) LacZ and interleukin 3 expression in vivo
after retroviral transduction of marrow derived human
osteogenic mesenchymal progenitors. Human Gene Therapy
8:1417-1427. Abstract
Velleman, S. G., McFarland, D. C., Li, Z., Ferrin, N.
H., Whitmoyer, R., and Dennis, J. E. (1997) Alterations
in sarcomere structure, collagen organization, mitochondrial
activity, and protein metabolism in the avian low score
normal muscle weakness. Dev. Growth Differ. 39:563-570.
Abstract
Hanada, K., Dennis, J. E., and Caplan, A. I. (1997) Stimulatory
effects of basic fibroblast growth factor (bFGF) and bone
morphogenetic protein 2 (BMP) on osteogenic differentiation
of rat bone marrow derived mesenchymal stem cells (MSCs).
J. Bone Min. Res. 12:1606-1614. Abstract
Caplan, A. I., Dennis, J. E. (1996) Mesenchymal stem cells:
Progenitors, progeny and pathways. J. Bone Min. Metab.
14:193-201. Abstract
Cassiede, P., Dennis, J. E., Ma, F., and Caplan, A. I.
(1996) Osteochondrogenic potential of marrow mesenchymal
progenitor cells exposed to TGFb 1 of PDGF BB as assayed
in vivo or in vitro. J. Bone Min. Res. 11:1-10. Abstract
Dennis, J. E., and Caplan, A.I. (1996) Analysis of the
developmental potential of conditionally immortal marrow
derived mesenchymal progenitor cells isolate from the
H2Kb A58 transgenic mouse. Conn. Tissue Res. 35:93-99.
Abstract
Dennis, J. E., and Caplan, A.I. (1996) Differentiation
potential of conditionally immortalized mesenchymal progenitor
cells from adult marrow of a H-2Kb-tsA58 transgenic mouse.
J. Cellular Physiol. 167:523-538. Abstract
Dennis, J. E. (1995) Mesenchymal Progenitors in Adult
Marrow. Ph. D. Thesis, Case Western Reserve University.
Dennis, J. E., Si Qun, S., Agarwal, M., Heuer, A., and
Caplan, A.I. (1995) Microstructure of Matrix and Mineral
Components of Eggshells From White Leghorn Chickens (Gallus
gallus). J. Morph. 228:287-306. Abstract
Saito, T., Dennis, J. E., Lennon, D.P., Young, R. G.,
and Caplan, A.I. (1995) Myogenic Expression of Mesenchymal
Stem Cells within Myotubes of mdx Mice In Vitro and In
Vivo. Tissue Eng. 4:327-343.
Lennon, D.P., Haynesworth, S.E., Young, R.G., Dennis,
J. E., and Caplan, A.I. (1995) A Chemically Defined Medium
Supports Proliferation and Maintains Osteochondrogenic
Potential of Rat Marrow Derived Mesenchymal Stem Cells.
Exp. Cell Res. 219:211-222. Abstract
Carrino, D.A., Dennis, J. E., Drushel, R. F., Haynesworth,
S.E., and Caplan, A.I. (1994) Identity of the Core Proteins
of the Large Chondroitin Sulfate Proteoglycans Synthesized
by Skeletal Muscle and Prechondrogenic Mesenchyme. Biochem.
J. 298:51-60. Abstract
Dennis, J. E., and Caplan, A.I. (1993) Porous Ceramic
Vehicles for Rat marrow derived (Rattus norvegicus) Osteogenic
Cell Delivery: Effects of Pre treatment with Fibronectin
or Laminin. Oral Implant. 19:106-115. Abstract
Dennis, J. E., Haynesworth, S.E., Young, R.G. and Caplan,
A.I. (1992) Osteogenesis in Marrow Derived Mesenchymal
Cell Porous Ceramic Composites Transplanted Subcutaneously:
Effect of Fibronectin and Laminin on Cell Retention and
Rate of Osteogenic Expression. Cell Transpl. 1:23-32.
Abstract
Han, Y., Dennis, J. E., Cohen Gould, L., Bader, D.M. and
Fischman, D.A. (1992) Expression of Sarcomeric Myosin
in the Presumptive Myocardium of Chicken Embryos Occurs
Within Six Hours of Myocyte Commitment. Dev. Dynam. 193:257-265.
Abstract
Arias, J.L., Carrino, D.A., Fernandez, M.S., Rodriguez,
J.P., Dennis, J. E., Caplan, A.I. (1992) Partial Biochemical
and Immunochemical Characterization of Avian Eggshell
Extracellular Matrices. Arch. Biochem. Biophys. 298:293-302.
Abstract
Nakahara, H., Dennis, J. E., Bruder, S.P., Haynesworth,
S.E., Lennon, D.P. and Caplan, A.I. (1991) In Vitro Differentiation
of Bone and Hypertrophic Cartilage From Periosteal Derived
Cells. Exp. Cell Res. 195:492-503. Abstract
Fernandez, M.S., Dennis, J. E., Drushel, R.F., Carrino,
D.A. and Caplan, A.I. (1991) The Dynamics of Compartmentalization
of Embryonic Muscle by Extracellular Matrix Molecules.
Dev. Biol. 147:46-61. Abstract
Arias, J.L., Fernandez, M.S., Dennis, J. E. and Caplan,
A.I. (1991) "The Fabrication and Collagenous Substructure
of the Eggshell Membrane in the Isthmus of the Hen Oviduct."
Matrix 11:313-320. Abstract
Arias, J.L., Fernandez, M.S., Dennis, J. E. and Caplan,
A.I. (1991) "Collagens of the Chicken Eggshell Membranes."
Conn. Tiss. Res. 26:37-45. Abstract
Saad, A.D., Dennis, J. E., Tan, I. and Fischman, D.A.
(1991) Visualization of myosin exchange between synthetic
thick filaments. J. Musc. Res. Cell Motil. 12:225-234.
Abstract
Nanduri, J., Dennis, J. E., Rosenberry, T.L., Mahmoud,
A.A.F. and Tartakoff, A.M. (1991) Glyocalyx of Bodies
versus Tails of Schistosoma Mansoni Cercariae: Lectin
Binding, Size, Charge and Electron Microscopic Characterization.
J. Biol. Chem. 266:1341 1347. Abstract
Dennis, J. E., Carrino, D.A., Schwartz, N.B., Caplan,
A.I. (1990) "Ultrastructural Characterization of
Embryonic Chick Cartilage Proteoglycan Core Protein and
the Mapping of a Monoclonal Antibody Epitope." J.
Biol. Chem. 265:12098-12103. Abstract
Peng, I., Dennis, J. E., Rodriguez Boulan, E. and Caplan,
A.I. (1990) Polarized Release of Enveloped Viruses in
the Embryonic Chick Heart: Demonstration of Epithelial
Polarity in the Presumptive Myocardium. Dev. Biol. 141:164-172.
Abstract
Shimizu, T., Dennis, J. E., Masaki, T. and Fischman, D.A.
(1985) Axial Arrangement of the Myosin Rod in Vertebrate
Thick Filaments: Immunoelectron Microscopy With a Monoclonal
Antibody to Light Meromyosin. J. Cell Biol. 101:1115-1123.
Abstract
Dennis, J. E., Shimizu, T., Reinach, F.C. and Fischman,
D.A. (1984) Localization of C Protein Isoforms in Chicken
Skeletal Muscles: Ultrastructural Detection Using Monoclonal
Antibodies. J. Cell Biol. 98:1514-1522. Abstract
Wallace, E., Calvin, H.I., Salfo, M.P., Dennis, J. E.
and Ploetz, K. (1984) Normal Levels of Zinc and Sulfhydryls
in Morphologically Abnormal Populations of Spermatozoa
From Moderately Zinc Deficient Rats. Gamete Res. 9:375-386.
Abstract
Jagiello, G.M., Dennis, J. E., Hiura, M. and Ducayen,
M. (1983). Incorporation of L 3H Fucose in the Rete and
Ovary of the Fetal Mouse. Gamete Res. I7:155-160. Abstract
Baum, S.G., Whittner, M., Nadler, J.P., Horowitz, S.B.,
Dennis, J. E., Schiff, P.B. and Tanowitz, H. (1981) Taxol,
A Microtubule Stabilizing Agent, Blocks the Replication
of Trypanosoma Cruzi. Proc. Natl Acad. Sci. USA 78:4571-4575.
Abstract
Hiura, M., Jagiello, G.M., Dennis, J. E. and Ducayen,
M. (1980) Ultracytochemical Localization of 3ß hydroxy
steroid Ferricyanide Reductase Activity in the Metal Mouse
Ovary. Gamete Res. 3:323-328. Abstract
INVITED SEMINAR PRESENTATIONS
- "Multipotentiality of Immortalized Mesenchymal
Stem Cells." Laboratoire d'anatomo pathologie, Hôpital
Jean Minjoz, Besançon, 1998.
- "The Application of Mesenchymal Stem Cells to Tissue
Repair." International Conference on Emerging Trends
in Biotechnology: Stem Cells technology, Potential and
Trends, All India Biotech Association, Hyderbad, India,
May, 2002.
- "Use of Mesenchymal Stem Cells in OTO HNS."
Research Advances in Otolaryngology, University Hospitals
of Cleveland, Cleveland, OH, June, 2002.
- "Human Mesenchymal Stem Cells for Cartilage repair.",
International Symposium on Mesenchymal Stem Cells, Valencia,
Spain, October, 2002.
- "The characterization of Mesenchymal Progenitor
Cells and their Application to Cartilage Repair.",
Association for Research in Otolaryngology, 26th Midwinter
Research Meeting, Special Symposium: Stem Cells and Progenitors:
Identification, Isolation, and Use, Daytona Beach, FL,
February, 2003.
- "Targeting of Progentitor Cells.", The Musculoskeletal
Research Seminar Series, Cleveland Clinic/Case, February
19, 2004.
- " Engineering of Cartilage and Bone " Seminar
at the Kinikum der Universit@t München, Klinik und
Poliklinik für Hals-Nasen-Ohren-Heilkinde-Grosshadern,
Munich, Germany, January 17, 2005.
- "Tissue Engineering of Cartilage and Bone"
Seminar at the Department of Orthopedics, Karolinska Universitetssjuhuset
at Huddinge, Sweden, January 20, 2005.
- "Engineering autologous cartilage for tracheal
reconstruction", First Annual Research Day, Department
of Otolaryngology-Head & Neck Surgery, March 12, 2005.
- "Strategies for Tissue Engineering of Cartilage",
Orthopaedics Grand Rounds, Case Western Reserve University,
June 22, 2005.
- "Clinical Application of Bioreactor-Expanded Osteoprogenitor
Cells", Genostem Satellite Symposium: Current trends
and new challenges in the regeneration of connective tissues,
Porto, Portugal, January 19, 2006.
- "Engineered Cartilage for Tracheal Reconstruction",
Annual Research Day, Department of Otolaryngology-Head
& Neck Surgery, March 18, 2006.
SERVICE -- PROFESSIONAL Reviewer for: Tissue Engineering,
Journal of Bone and Mineral Research, Experimental Hematology,
European Journal of Biochemistry, Stem Cells, Bone, Experimental
Cell Research, Laboratory Investigation, Journal of Orthopedic
Research, Cells Tissues Organs, and Matrix.
Professional Affiliation(s):
American Society for Bone and Mineral Research, Orthopaedic
Research Society
Scientific Advisory Board for Genostem (A non-profit consortium
of European scientists studying mesenchymal stem cells)
RO3 Grant Reviewer for National Institute of Arthritis
and Musculoskeletal and Skin Diseases (September, 2005;
December, 2005).
Grant Reviewer for the Biomedical Research Council, Singapore,
2005
Arthritis and Musculoskeletal and Skin Diseases Special
Grants Review Committee, 2006
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