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Biochemistry Department - Primary Faculty

Marion J. Skalweit, M.D. Ph.D.

Assistant Professor of Medicine and Biochemistry, Case Western Reserve University
Staff Physician, Internal Medicine and Infectious Diseases, Louis Stokes Cleveland Department of Veterans Affairs Medical Center

Education

  • Ph.D.: in Chemistry (1989) from University of California at Berkeley
  • M.D.: (1996) from Case Western Reserve University
  • B.S.: in Chemistry (1979) from Case Western Reserve University

Research Interests

My laboratory is interested in resistance to beta-lactam antibiotics and beta-lactamase inhibitors caused by a wide variety of beta-lactamase enzymes such as Class A beta-lactamases (SHV of Klebsiella pneumoniae; TEM of E. coli); Class C beta-lactamases (CMY-2 enzyme) and Class B metallo-beta-lactamases (VIM-2 of Pseudomonas aeruginosa). In addition, we are studying the basis of sulbactam as an antibiotic for Acinetobacter baumannii and the role of metallo-beta-lactamase like enzymes in biofilm formation by a variety of bacteria. We employ a variety of microbiologic, molecular biologic, epidemiologic and biophysical methods to study resistance phenomena where they occur in the hospital and the laboratory.

Selected References

  • Drawz S. M., Bethel C. R., Doppalapudi V. R., Sheri A., Pagadala S. R., Hujer A. M., Skalweit M. J., Anderson V. E., Chen S. G., Buynak J. D., and Bonomo R. A.
    “Penicillin sulfone inhibitors of class D beta-lactamases”
    Antimicrob Agents Chemother 54 (4): 1414-24 (2010). Read article in PubMedCentral
  • Endimiani A., Doi Y., Bethel C. R., Taracila M., Adams-Haduch J. M., O'Keefe A., Hujer A. M., Paterson D. L., Skalweit M. J., Page M. G., Drawz S. M., and Bonomo R. A.
    “Enhancing resistance to cephalosporins in class C beta-lactamases: impact of Gly214Glu in CMY-2”
    Biochemistry 49 (5): 1014-23 (2010).
  • Nixdorff N. A., Tang J., Mourad R., and Skalweit M. J.
    “SAME is different: a case report and literature review of Staphylococcus aureus metastatic endophthalmitis”
    South Med J 102 (9): 952-6 (2009).
  • Bethel C. R., Distler A. M., Ruszczycky M. W., Carey M. P., Carey P. R., Hujer A. M., Taracila M., Helfand M. S., Thomson J. M., Kalp M., Anderson V. E., Leonard D. A., Hujer K. M., Abe T., Venkatesan A. M., Mansour T. S., and Bonomo R. A.
    “Inhibition of OXA-1 beta-lactamase by penems”
    Antimicrob Agents Chemother 52 (9): 3135-43 (2008). Read article in PubMedCentral
  • Helfand M. S., Taracila M. A., Totir M. A., Bonomo R. A., Buynak J. D., van den Akker F., and Carey P. R.
    “Raman crystallographic studies of the intermediates formed by Ser130Gly SHV, a beta-lactamase that confers resistance to clinical inhibitors”
    Biochemistry 46 (29): 8689-99 (2007).
  • Miani A., Raugei S., Carloni P., and Helfand M. S.
    “Structure and Raman spectrum of clavulanic acid in aqueous solution”
    J Phys Chem B 111 (10): 2621-30 (2007).
  • Totir M. A., Helfand M. S., Carey M. P., Sheri A., Buynak J. D., Bonomo R. A., and Carey P. R.
    “Sulbactam forms only minimal amounts of irreversible acrylate-enzyme with SHV-1 beta-lactamase”
    Biochemistry 46 (31): 8980-7 (2007). Read article in PubMedCentral
  • Bethel C. R., Hujer A. M., Hujer K. M., Thomson J. M., Ruszczycky M. W., Anderson V. E., Pusztai-Carey M., Taracila M., Helfand M. S., and Bonomo R. A.
    “Role of Asp104 in the SHV beta-lactamase”
    Antimicrob Agents Chemother 50 (12): 4124-31 (2006). Read article in PubMedCentral
  • Helfand M. S. and Bonomo R. A.
    “Extended-spectrum beta-lactamases in multidrug-resistant Escherichia coli: changing the therapy for hospital-acquired and community-acquired infections”
    Clin Infect Dis 43 (11): 1415-6 (2006).
  • Padayatti P. S., Sheri A., Totir M. A., Helfand M. S., Carey M. P., Anderson V. E., Carey P. R., Bethel C. R., Bonomo R. A., Buynak J. D., and van den Akker F.
    “Rational design of a beta-lactamase inhibitor achieved via stabilization of the trans-enamine intermediate: 1.28 A crystal structure of wt SHV-1 complex with a penam sulfone”
    J Am Chem Soc 128 (40): 13235-42 (2006). Read article in PubMedCentral
  • Totir M. A., Padayatti P. S., Helfand M. S., Carey M. P., Bonomo R. A., Carey P. R., and van den Akker F.
    “Effect of the inhibitor-resistant M69V substitution on the structures and populations of trans-enamine beta-lactamase intermediates”
    Biochemistry 45 (39): 11895-904 (2006). Read article in PubMedCentral
  • Stiefel U., Harmoinen J., Koski P., Kaariainen S., Wickstrand N., Lindevall K., Pultz N. J., Bonomo R. A., Helfand M. S., and Donskey C. J.
    “Orally administered recombinant metallo-beta-lactamase preserves colonization resistance of piperacillin-tazobactam-treated mice”
    Antimicrob Agents Chemother 49 (12): 5190-1 (2005). Read article in PubMedCentral

 

Marion Skalweit Faculty's publications at pubmed more in the biochembook