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Center for Inherited Disorders of Energy Metabolism

 

Fresh Muscle Biopsies at Cleveland, OH

 

Below are assays that can be ordered when a fresh muscle biopsy is done in Cleveland, Ohio. For a comprehensive list that includes CPT codes and costs see Test and Price List for On-Site Muscle Biopsy Studies.

Test Method Description CPT Code
Adenine Nucleotide Translocase
Spectrophotometric enzyme assays
84999

 

Test Method Description CPT Code
Carnitine Palmitoyltransferases (I & II)
Three radioactive enzyme assays of total CPT activity: (1) forward direction, (2) reverse reaction, and (3) malonyl-CoA sensitive activity, forward reaction
84999

 

Test Method Description CPT Codes

Electron Transport Chain (Complexes I-IV)

Spectrophotometric enzyme assays: cytochrome c reduction with NADH + rotenone (I + III), succinate + antimycin (II +III), decylubiquinol + antimycin (III), oxidation of reduced cytochrome c (IV)
84999 82657 84157 80502

 

Test Method Description CPT Codes
Fatty Acid Oxidation Enzymes

Spectrophotometric enzyme assays with chain-length specific substrates (intermediates of b-oxidation)

84999 82657 84157 80502

 

Test Method Description CPT Codes
a-Ketoglutarate Dehydrogenase Complex
Radioactive enzyme assay

84999, 82657 84157, 80502

 

Test Method Description CPT Code

Krebs cycle enzymes

*Aconitase *Fumarase *Glutamate Dehydrogenase *Isocitrate Dehydrogenase *Malate Dehydrogenase *Succinate Dehydrogenase

Spectrophotometric or radioactive enzyme assays
84999

 

Test Method Description CPT Code
Oxidative Phosphorylation
Isolation of mitochondria from fresh tissue with assays of oxidative phosphorylation using multiple substrates to study overall mitochondrial function
84999

 

Test Method Description CPT Codes
Pyruvate Dehydrogenase Complex
Radioactive enzyme assay by 1-14C-pyruvate decarboxylation (includes components if overall activity low) and dihydrolipoamide dehydrogenase
84999 82657 84157 80502

 

 

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