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Center for Inherited Disorders of Energy Metabolism

 

b-HYDROXYBUTYRATE/ACETOACETATE

 

Indications: For diagnostic testing of fasting hypoglycemia or after an oral fat tolerance test. The ratio of b-hydroxybutyrate/acetoacetate(AcAc) reflects mitochondrial NAD/NADH, and may be a useful parameter for diagnosis of defects of the Krebs cycle.

 

Method Description: Spectrophotometric enzymatic assays are done to measure the b-hydroxybutyrate/AcAc in plasma as a parameter of lipolyis and fatty acid oxidation or to monitor efficacy of ketogenic diets.

Specimen
Specimen Requirements
Shipping Requirements
Plasma

Collect 0.5-1 ml blood in an EDTA tube (lavender top), mix gently, and centrifuge as soon as possible after collection. Then transfer the plasma into a polypropylene tube and freeze.

Minimum Amt Required: 200 µl

Ship sample on dry ice.

Samples Accepted: Monday - Friday, 8:30 AM - 5:00 PM

Turn-Around-Time: 5-7 business days

CPT Code(s): 82010, 80500

Specimens can be sent via Federal Express, DHL, or UPS.

Special Instructions: Ship using Priority Overnight service.

 

 

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CIDEM | University Hospitals Case Medical Center | 11100 Euclid Avenue, WEARN Rm 649
Cleveland, Ohio 44106 | Phone: 216.844.1286 | Fax: 216-844-8005

© 2004 Case Western Reserve University | Cleveland, Ohio 44106 | 216.368.2000 | legal notice | Updated:3/10/08