FACULTY & STAFF
James D. Burgess
Associate Professor of Chemistry
jdb22@case.edu
Electrochemistry
B.S. Longwood College, 1992
Ph.D. Virginia Commonwealth University, 1997 Postdoctoral, Iowa
State University, 1998
Research focuses on the modification of electrode surfaces with biological molecules for both sensing and fundamental studies. Current areas of interest include electrochemical detection of cholesterol efflux from single living cells and reactions of platinum based anticancer drugs with DNA.
Characterization of the cholesterol content of cell plasma membrane
The aim of this research is to develop electrochemical methodology that will investigate the cholesterol content of cell plasma membranes. The ability to evaluate the dynamics of intracellular cholesterol trafficking to and from the plasma membrane would allow characterization of pathways governing cholesterol homeostasis and, in particular, of the initial steps in atherogenesis. Our group has established a procedure for fabrication of an electrode-supported lipid bilayer membrane that allows immobilization of cholesterol oxidase, which could be employed to detect the cholesterol contained in the plasma membrane of a single cell. The steady-state electrode response observed appears to correlate with the cholesterol content of plasma membrane.
Handling single cells.
DNA Binding of Platinum Based Anticancer Drugs
The aim of this research is to probe, in real time, the contributions of electrostatic and hydrogen bonding interactions to the sequence specificity of platinum binding to DNA and RNA. The binding rates are being studied using a quartz crystal microbalance (QCM) modified with a target sequence. This approach is also well suited for evaluating the recognition of DNA-platinum adducts by biomolecules possibly involved in repair of damaged DNA and drug resistance.
Selected Publications
- Dechen Jiang, Anando Devadoss, Mariela S. Palencsár, D. Fang, Nicole M. White, Thomas J. Kelley, J. Smith, and James D. Burgess. "Direct Electrochemical Evaluation of Plasma Membrane Cholesterol in live Mammalian Cells." J. Am. Chem. Soc. 2007, 129: 11352-11353
- Nicole, M. White, Dechen Jiang, James D. Burgess, Ilya R. Bederman, Stephen F. Previs, Thomas J. Kelley. "Altered cholesterol homeostasis in cultured and in vivo models of cystic fibrosis." American Journal of Physiology-Lung Cellular and Molecular Physiology. 2007, 292: L476-L486.
- Michael P. Bokoch, Anando Devadoss, Mariela S. Palencsár, James D. Burgess. "Steady-State Oxidation of Cholesterol Catalyzed by Cholesterol Oxidase in Lipid Bilayer Membranes on Platinum Electrodes." Analytica Chimica Acta. 2004, 519: 47-55
- Anando Devadoss and James D. Burgess. "Steady-State Detection of Cholesterol Contained in the Plasma Membrane of a Single Cell Using Lipid Bilayer-Modified Microelectrodes Incorporating Cholesterol Oxidase." J. Am. Chem. Soc. 2004, 126: 10214-10215
- Jonathan S. Steckel, Nicole S. Persky, Chelsea R. Martinez, Calvin L. Barnes, Elizabeth A. Fry, Jaideep Kulkarni, James D. Burgess, Rachel B. Pacheco, and Sarah L. Stoll. "Monolayer and Multilayer Films of [Mn12O12(O2CMe)16]." Nano Letters. 2004, 4(3): 399-402
- Melissa C. Rhoten, James D. Burgess, and Fred M. Hawkridge. "The Reaction of Cytochrome C From Different Species With Cytochrome C Oxidase Immobilized in an Electrode Supported Lipid Bilayer Membrane." J. Electroanalytical Chem. 2002, 534: 143-150
- James D. Burgess and Fred M. Hawkridge. "Direct Electron Transfer Reactions of Proteins and Enzymes at Electrodes". In Electroanalytical Methods of Biological Materials. A. Bajter-Toth and J.Q. Chambers, Eds. 2002.
- Anando Devadoss and James D. Burgess. "Detection of Cholesterol through Electron Transfer to Cholesterol Oxidase in Electrode-Supported Lipid Bilayer Membranes." Langmuir. 2002, 18: 9617-9621
- Yueju Wang, Nicholas Farrell, and James D. Burgess. "Direct Evidence for Preassociation Preceding Covalent Binding in the Reaction of cis-[Pt(NH3)2(H2O)2]2+ with Surface Immobilized Oligonucleotides." J. Am. Chem. Soc. 2001, 123: 5576-5577